SrasV1, Main, Exploration, bibRecord, 002948

Biflavonoids from Torreya nucifera displaying SARS-CoV 3CLpro inhibition

Identifieur interne : 002948 ( Main/Exploration ); précédent : 002947; suivant : 002949

Biflavonoids from Torreya nucifera displaying SARS-CoV 3CLpro inhibition

Auteurs : YOUNG BAE RYU [Corée du Sud] ; HYUNG JAE JEONG [Corée du Sud] ; JANG HOON KIM [Corée du Sud] ; YOUNG MIN KIM [Corée du Sud] ; Ji-Young Park [Corée du Sud] ; Doman Kim [Corée du Sud] ; Thi Thanh Hanh Naguyen [Corée du Sud] ; Su-Jin Park [Corée du Sud] ; JONG SUN CHANG [Corée du Sud] ; KI HUN PARK [Corée du Sud] ; Mun-Chual Rho [Corée du Sud] ; WOO SONG LEE [Corée du Sud]

Source :

Bioorganic & medicinal chemistry [ 0968-0896 ] ; 2010.

RBID : Pascal:11-0215097

Descripteurs français

Pascal (Inist)
- Flavonoïde, Virus syndrome respiratoire aigu sévère, Isolement, Antiviral, Inhibiteur enzyme, Cysteine endopeptidases, Médecine orientale, Dérivé de la flavone, Relation structure activité, Plante médicinale, Pharmacognosie, In vitro, Modèle moléculaire, Complexe enzyme inhibiteur, Modélisation, Coniferales, Médecine traditionnelle, Asie, Origine végétale, Amentoflavone, Torreya nucifera.
Wicri :
- topic : Plante médicinale.

English descriptors

KwdEn :
- Antiviral, Asia, Coniferales, Cysteine endopeptidases, Enzyme inhibitor, Flavone derivatives, Flavonoid, Folk medicine, In vitro, Inhibitor enzyme complex, Isolation, Medicinal plant, Modeling, Molecular model, Oriental medicine, Pharmacognosy, Plant origin, Severe acute respiratory syndrome virus, Structure activity relation.

Abstract

As part of our search for botanical sources of SARS-CoV 3CL^pro inhibitors, we selected Torreya nucifera, which is traditionally used as a medicinal plant in Asia. The ethanol extract of T. nucifera leaves exhibited good SARS-CoV 3CL^pro inhibitory activity (62% at 100 μg/mL). Following bioactivity-guided fractionation, eight diterpenoids (1-8) and four biflavonoids (9-12) were isolated and evaluated for SARS-CoV 3CL^pro inhibition using fluorescence resonance energy transfer analysis. Of these compounds, the biflavone amentoflavone (9) (IC₅₀ ₌ 8.3 μM) showed most potent 3CL^pro inhibitory effect. Three additional authentic flavones (apigenin, luteolin and quercetin) were tested to establish the basic structure-activity relationship of biflavones. Apigenin, luteolin, and quercetin inhibited 3CL^pro activity with IC₅₀ values of 280.8, 20.2, and 23.8 μM, respectively. Values of binding energy obtained in a molecular docking study supported the results of enzymatic assays. More potent activity appeared to be associated with the presence of an apigenin moiety at position C-3' of flavones, as biflavone had an effect on 3CL^pro inhibitory activity. .

Affiliations:

Corée du Sud

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Woo Song Lee" sort="Woo Song Lee" uniqKey="Woo Song Lee" last="Woo Song Lee">WOO SONG LEE</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral</term>
<term>Asia</term>
<term>Coniferales</term>
<term>Cysteine endopeptidases</term>
<term>Enzyme inhibitor</term>
<term>Flavone derivatives</term>
<term>Flavonoid</term>
<term>Folk medicine</term>
<term>In vitro</term>
<term>Inhibitor enzyme complex</term>
<term>Isolation</term>
<term>Medicinal plant</term>
<term>Modeling</term>
<term>Molecular model</term>
<term>Oriental medicine</term>
<term>Pharmacognosy</term>
<term>Plant origin</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Structure activity relation</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Flavonoïde</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Isolement</term>
<term>Antiviral</term>
<term>Inhibiteur enzyme</term>
<term>Cysteine endopeptidases</term>
<term>Médecine orientale</term>
<term>Dérivé de la flavone</term>
<term>Relation structure activité</term>
<term>Plante médicinale</term>
<term>Pharmacognosie</term>
<term>In vitro</term>
<term>Modèle moléculaire</term>
<term>Complexe enzyme inhibiteur</term>
<term>Modélisation</term>
<term>Coniferales</term>
<term>Médecine traditionnelle</term>
<term>Asie</term>
<term>Origine végétale</term>
<term>Amentoflavone</term>
<term>Torreya nucifera</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante médicinale</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">As part of our search for botanical sources of SARS-CoV 3CL<sup>pro</sup>
 inhibitors, we selected Torreya nucifera, which is traditionally used as a medicinal plant in Asia. The ethanol extract of T. nucifera leaves exhibited good SARS-CoV 3CL<sup>pro</sup>
 inhibitory activity (62% at 100 μg/mL). Following bioactivity-guided fractionation, eight diterpenoids (1-8) and four biflavonoids (9-12) were isolated and evaluated for SARS-CoV 3CL<sup>pro</sup>
 inhibition using fluorescence resonance energy transfer analysis. Of these compounds, the biflavone amentoflavone (9) (IC<sub>50</sub>
 <sub>=</sub>
 8.3 μM) showed most potent 3CL<sup>pro</sup>
 inhibitory effect. Three additional authentic flavones (apigenin, luteolin and quercetin) were tested to establish the basic structure-activity relationship of biflavones. Apigenin, luteolin, and quercetin inhibited 3CL<sup>pro</sup>
 activity with IC<sub>50</sub>
 values of 280.8, 20.2, and 23.8 μM, respectively. Values of binding energy obtained in a molecular docking study supported the results of enzymatic assays. More potent activity appeared to be associated with the presence of an apigenin moiety at position C-3' of flavones, as biflavone had an effect on 3CL<sup>pro</sup>
 inhibitory activity. .</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
</country>
</list>
<tree><country name="Corée du Sud"><noRegion><name sortKey="Young Bae Ryu" sort="Young Bae Ryu" uniqKey="Young Bae Ryu" last="Young Bae Ryu">YOUNG BAE RYU</name>
</noRegion>
<name sortKey="Hyung Jae Jeong" sort="Hyung Jae Jeong" uniqKey="Hyung Jae Jeong" last="Hyung Jae Jeong">HYUNG JAE JEONG</name>
<name sortKey="Jang Hoon Kim" sort="Jang Hoon Kim" uniqKey="Jang Hoon Kim" last="Jang Hoon Kim">JANG HOON KIM</name>
<name sortKey="Jong Sun Chang" sort="Jong Sun Chang" uniqKey="Jong Sun Chang" last="Jong Sun Chang">JONG SUN CHANG</name>
<name sortKey="Ki Hun Park" sort="Ki Hun Park" uniqKey="Ki Hun Park" last="Ki Hun Park">KI HUN PARK</name>
<name sortKey="Kim, Doman" sort="Kim, Doman" uniqKey="Kim D" first="Doman" last="Kim">Doman Kim</name>
<name sortKey="Naguyen, Thi Thanh Hanh" sort="Naguyen, Thi Thanh Hanh" uniqKey="Naguyen T" first="Thi Thanh Hanh" last="Naguyen">Thi Thanh Hanh Naguyen</name>
<name sortKey="Park, Ji Young" sort="Park, Ji Young" uniqKey="Park J" first="Ji-Young" last="Park">Ji-Young Park</name>
<name sortKey="Park, Su Jin" sort="Park, Su Jin" uniqKey="Park S" first="Su-Jin" last="Park">Su-Jin Park</name>
<name sortKey="Rho, Mun Chual" sort="Rho, Mun Chual" uniqKey="Rho M" first="Mun-Chual" last="Rho">Mun-Chual Rho</name>
<name sortKey="Woo Song Lee" sort="Woo Song Lee" uniqKey="Woo Song Lee" last="Woo Song Lee">WOO SONG LEE</name>
<name sortKey="Young Min Kim" sort="Young Min Kim" uniqKey="Young Min Kim" last="Young Min Kim">YOUNG MIN KIM</name>
</country>
</tree>
</affiliations>
</record>

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Biflavonoids from Torreya nucifera displaying SARS-CoV 3CLpro inhibition

Biflavonoids from Torreya nucifera displaying SARS-CoV 3CLpro inhibition

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